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  • Featured Product
  • KD/KO Validated

SERPINB10 Polyclonal antibody

SERPINB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21719-1-AP

Print datasheet

Synonyms

bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10

验证数据展示

  • WB analysis using 21719-1-AP

    WB analysis using 21719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21719-1-AP

    IF Staining of HepG2 using 21719-1-AP

    Immunofluorescent analysis of HepG2 cells, using SERPINB10 antibody 21719-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human tonsillitis using 21719-1-AP

    IHC staining of human tonsillitis using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21719-1-AP (SERPINB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HL-60 cells, Sp2/0 cells, human saliva
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21719-1-AP is SERPINB10 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SERPINB10 fusion protein Ag16372 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 10
Synonyms bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC096217
Gene Symbol SERPINB10
Gene ID (NCBI) 5273
RRIDAB_10734592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48595
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINB10 antibody 21719-1-APDownload protocol
IHC protocol for SERPINB10 antibody 21719-1-APDownload protocol
IF protocol for SERPINB10 antibody 21719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Am J Physiol Lung Cell Mol Physiol

Epithelial SERPINB10, a novel marker of airway eosinophilia in asthma, contributes to allergic airway inflammation.

Authors - Yuqing Mo
  • KD Validated
View Article