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SERPINB13 Polyclonal antibody

SERPINB13 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 18045-1-AP

Print datasheet

Synonyms

Serpin B13, Peptidase inhibitor 13, Hurpin, HUR7, headpin

验证数据展示

  • IHC staining of human oesophagus cancer using 18045-1-AP

    IHC staining of human oesophagus cancer using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 18045-1-AP

    IHC staining of human oesophagus cancer using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18045-1-AP

    IF Staining of HepG2 using 18045-1-AP

    Immunofluorescent analysis of HepG2 cells, using SERPINB13 antibody 18045-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse skin using 18045-1-AP

    IHC staining of mouse skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 18045-1-AP

    IHC staining of mouse skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18045-1-AP (SERPINB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 18045-1-AP

    FC experiment of HepG2 using 18045-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SERPINB13 Polyclonal antibody (18045-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human skin using 18045-1-AP

    IHC staining of human skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 18045-1-AP

    IHC staining of human skin using 18045-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18045-1-AP (SERPINB13 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman oesophagus cancer tissue, mouse skin tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18045-1-AP targets SERPINB13 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SERPINB13 fusion protein Ag12686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 13
Synonyms Serpin B13, Peptidase inhibitor 13, Hurpin, HUR7, headpin
Calculated Molecular Weight 391 aa, 44 kDa
GenBank Accession NumberBC101821
Gene Symbol SERPINB13
Gene ID (NCBI) 5275
RRIDAB_2186160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SERPINB13 antibody 18045-1-APDownload protocol
IF protocol for SERPINB13 antibody 18045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols