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验证数据展示

  • WB analysis using 11428-1-AP

    WB analysis using 11428-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SerpinB3/SerpinB4 Polyclonal antibody

SerpinB3/SerpinB4 Polyclonal Antibody for WB, ELISA
Cat No. 11428-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, human saliva

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11428-1-AP targets SerpinB3/SerpinB4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SerpinB3/SerpinB4 fusion protein Ag1964 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 4
别名 LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4
计算分子量 390 aa, 45 kDa
观测分子量42-45 kDa
GenBank蛋白编号BC017401
基因名称 SERPINB4
Gene ID (NCBI) 6318
RRIDAB_3085373
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.

实验方案

Product Specific Protocols
WB protocol for SerpinB3/SerpinB4 antibody 11428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols