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SerpinB3/SerpinB4 Polyclonal antibody

SerpinB3/SerpinB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11428-1-AP

Print datasheet

Synonyms

LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4

验证数据展示

  • WB analysis using 11428-1-AP

    WB analysis using 11428-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, human saliva

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11428-1-AP targets SerpinB3/SerpinB4 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen SerpinB3/SerpinB4 fusion protein Ag1964 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 4
Synonyms LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4
Calculated Molecular Weight 390 aa, 45 kDa
Observed Molecular Weight42-45 kDa
GenBank Accession NumberBC017401
Gene Symbol SERPINB4
Gene ID (NCBI) 6318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.

实验方案

Product Specific Protocols
WB protocol for SerpinB3/SerpinB4 antibody 11428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols