验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, mouse skeletal muscle tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:18000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
14962-1-AP targets SERPINB6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SERPINB6 fusion protein Ag6824 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade B (ovalbumin), member 6 |
| 别名 | PI6, PTI, Serpin B6, PI-6, PI 6 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC001394 |
| 基因名称 | SERPINB6 |
| Gene ID (NCBI) | 5269 |
| RRID | AB_2186456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| IHC protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| IF protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| IP protocol for SERPINB6 antibody 14962-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Tissue Res Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouse submandibular gland. | ||
Front Neurol Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein. | ||
PLoS One New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. | ||
Cell Mol Gastroenterol Hepatol Single-cell transcriptomics reveals early molecular and immune alterations underlying the serrated neoplasia pathway toward colorectal cancer |
