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验证数据展示

  • WB analysis of Jurkat using 13102-2-AP

    WB analysis of Jurkat using 13102-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis using 13102-2-AP

    WB analysis using 13102-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13102-2-AP (SERPINB8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 13102-2-AP

    IHC staining of human oesophagus cancer using 13102-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINB8 antibody included
  • IHC staining of human oesophagus cancer using 13102-2-AP

    IHC staining of human oesophagus cancer using 13102-2-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13102-2-AP (SERPINB8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINB8 antibody included
  • Featured Product
  • KD/KO验证

SERPINB8 Polyclonal antibody

SERPINB8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13102-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PI8, Serpin B8, PI 8, Peptidase inhibitor 8, Cytoplasmic antiproteinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse lung tissue
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SERPINB8 Ready-To-Use IHC Kit. SERPINB8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13102-2-AP targets SERPINB8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 SERPINB8 fusion protein Ag3740 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 8
别名 PI8, Serpin B8, PI 8, Peptidase inhibitor 8, Cytoplasmic antiproteinase 2
计算分子量 374 aa, 43 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC034528
基因名称 SERPINB8
Gene ID (NCBI) 5271
RRIDAB_2186472
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPINB8 antibody 13102-2-APDownload protocol
IHC protocol for SERPINB8 antibody 13102-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Dermatol

SERPINB8 and furin regulate ITGAX expression and affect the proliferation and invasion of melanoma cells

Authors - Li Ni
  • KD Validated
View Article