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验证数据展示

  • WB analysis of K-562 using 67950-1-Ig (same clone as 67950-1-PBS)

    WB analysis of K-562 using 67950-1-Ig (same clone as 67950-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

    IHC staining of mouse lung using 67950-1-Ig (same clone as 67950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 67950-1-Ig (same clone as 67950-1-PBS)

    IF Staining of C6 using 67950-1-Ig (same clone as 67950-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67950-1-PBS in a different storage buffer formulation.

SERPINB9 Monoclonal antibody, PBS Only

SERPINB9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67950-1-PBS

产品说明书

CloneNo. 2H9D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67950-1-PBS targets SERPINB9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 SERPINB9 fusion protein Ag6719 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 9
别名 CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9
计算分子量 42 kDa
观测分子量38 kDa
GenBank蛋白编号BC002538
基因名称 SERPINB9
Gene ID (NCBI) 5272
RRIDAB_2918702
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP50453
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.