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验证数据展示

  • WB analysis using 67602-1-Ig

    WB analysis using 67602-1-Ig

    0.8 μL, 0.4 μL, and 0.2 μL human plasma lysates were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67602-1-Ig

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67602-1-Ig

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67602-1-Ig

    IF Staining of HepG2 using 67602-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPIND1 antibody (67602-1-Ig, Clone: 2C1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SERPIND1 Monoclonal antibody

SERPIND1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67602-1-Ig

产品说明书

CloneNo. 2C1E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma, human placenta tissue, pig plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67602-1-Ig targets SERPIND1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig
免疫原 SERPIND1 fusion protein Ag26351 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade D (heparin cofactor), member 1
别名 D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1
计算分子量 499 aa, 57 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC035028
基因名称 SERPIND1
Gene ID (NCBI) 3053
RRIDAB_2882808
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05546
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IHC protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IF protocol for SERPIND1 antibody 67602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols