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SERPIND1 Monoclonal antibody

SERPIND1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C1E1

Cat no : 67602-1-Ig

Print datasheet

Synonyms

D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1

验证数据展示

  • WB analysis using 67602-1-Ig

    WB analysis using 67602-1-Ig

    0.8 μL, 0.4 μL, and 0.2 μL human plasma lysates were subjected to SDS PAGE followed by western blot with 67602-1-Ig (SERPIND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67602-1-Ig

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67602-1-Ig

    IHC staining of human liver using 67602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67602-1-Ig (SERPIND1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67602-1-Ig

    IF Staining of HepG2 using 67602-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPIND1 antibody (67602-1-Ig, Clone: 2C1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma, human placenta tissue, pig plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67602-1-Ig targets SERPIND1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, pig
Immunogen SERPIND1 fusion protein Ag26351 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade D (heparin cofactor), member 1
Synonyms D22S673, HC II, HC2, HCF2, HCII, Heparin cofactor 2, Heparin cofactor II, HLS2, LS2, Protease inhibitor leuserpin 2, Serpin D1, SERPIND1
Calculated Molecular Weight 499 aa, 57 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC035028
Gene Symbol SERPIND1
Gene ID (NCBI) 3053
RRIDAB_2882808
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05546
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IHC protocol for SERPIND1 antibody 67602-1-IgDownload protocol
IF protocol for SERPIND1 antibody 67602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols