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验证数据展示

  • WB analysis of HepG2 using 13228-1-AP

    WB analysis of HepG2 using 13228-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13228-1-AP (Alpha-2-antiplasmin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13228-1-AP

    IF Staining of HepG2 using 13228-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 2-Antiplasmin antibody (13228-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Alpha 2-Antiplasmin Polyclonal antibody

Alpha 2-Antiplasmin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13228-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13228-1-AP targets Alpha 2-Antiplasmin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Alpha 2-Antiplasmin fusion protein Ag4095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
别名 Alpha-2-antiplasmin, Serpin F2, ALPHA 2 PI, Alpha 2 AP, Alpha 2 antiplasmin
计算分子量 491 aa, 55 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC031592
基因名称 Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2187194
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
IF protocol for Alpha 2-Antiplasmin antibody 13228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dis Markers

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Authors - Ting He
View Article
humanWB

Res Pract Thromb Haemost

Citrullination of α2-antiplasmin is unlikely to contribute to enhanced plasmin generation in COVID-19 pathophysiology

Authors - Kadri Kangro
View Article