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Neuroserpin Polyclonal antibody

Neuroserpin Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 12558-1-AP

Print datasheet

Synonyms

Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12

验证数据展示

  • WB analysis of mouse brain using 12558-1-AP

    WB analysis of mouse brain using 12558-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12558-1-AP (Neuroserpin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 12558-1-AP

    IP experiment of rat brain using 12558-1-AP

    IP result of anti-Neuroserpin (IP:12558-1-AP, 4ug; Detection:12558-1-AP 1:300) with rat brain tissue lysate 1880 ug.

  • IF Staining of mouse brain using 12558-1-AP

    IF Staining of mouse brain using 12558-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Neuroserpin antibody (12558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inrat brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12558-1-AP targets Neuroserpin in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Neuroserpin fusion protein Ag3275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade I (neuroserpin), member 1
Synonyms Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC018043
Gene Symbol Neuroserpin
Gene ID (NCBI) 5274
RRIDAB_10642703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.

实验方案

Product Specific Protocols
WB protocol for Neuroserpin antibody 12558-1-APDownload protocol
IF protocol for Neuroserpin antibody 12558-1-APDownload protocol
IP protocol for Neuroserpin antibody 12558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols