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验证数据展示

  • WB analysis of mouse brain using 12558-1-AP

    WB analysis of mouse brain using 12558-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12558-1-AP (Neuroserpin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 12558-1-AP

    IP experiment of rat brain using 12558-1-AP

    IP result of anti-Neuroserpin (IP:12558-1-AP, 4ug; Detection:12558-1-AP 1:300) with rat brain tissue lysate 1880 ug.

  • IF Staining of mouse brain using 12558-1-AP

    IF Staining of mouse brain using 12558-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Neuroserpin antibody (12558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Neuroserpin Polyclonal antibody

Neuroserpin Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 12558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, ELISA

别名

Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inrat brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12558-1-AP targets Neuroserpin in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Neuroserpin fusion protein Ag3275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade I (neuroserpin), member 1
别名 Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12
计算分子量 410 aa, 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC018043
基因名称 Neuroserpin
Gene ID (NCBI) 5274
RRIDAB_10642703
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99574
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.

实验方案

Product Specific Protocols
WB protocol for Neuroserpin antibody 12558-1-APDownload protocol
IF protocol for Neuroserpin antibody 12558-1-APDownload protocol
IP protocol for Neuroserpin antibody 12558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols