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Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21346-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA and More (1)

SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue, HeLa cells, K-562 cells, mouse lung tissue, NIH/3T3 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21346-1-AP targets Sestrin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Sestrin 2 fusion protein Ag15669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sestrin 2
别名 SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene
计算分子量 480 aa, 54 kDa
观测分子量 60-66 kDa
GenBank蛋白编号NM_031459
基因名称 SESN2/Sestrin 2
Gene ID (NCBI) 83667
RRIDAB_10733238
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

实验方案

Product Specific Protocols
WB protocol for Sestrin 2 antibody 21346-1-APDownload protocol
IF protocol for Sestrin 2 antibody 21346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
humanWB

Elife

Dynamics of mTORC1 activation in response to amino acids.

Authors - Maria Manifava
  • KD Validated
mouseWB,IF

Front Immunol

Sestrin2 provides cerebral protection through activation of Nrf2 signaling in microglia following subarachnoid hemorrhage

Authors - Youqing Yang
humanWB,IF

J Invest Dermatol

Metastatic melanoma cells rely on Sestrin2 to acquire anoikis resistance via detoxifying intracellular ROS.

Authors - Guannan Zhu
  • KD Validated
ratWB

Mol Cell Biol

Sestrin2 protects dopaminergic cells against rotenone toxicity through AMPK-dependent autophagy activation.

Authors - Yi-Sheng Hou
ratIF

J Cereb Blood Flow Metab

Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups.

Authors - Xudan Shi
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