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SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB, ELISA
Cat No. 11431-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (1)

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SESN3 fusion protein Ag1961 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sestrin 3
别名 Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-
计算分子量 492 aa, 57 kDa
观测分子量 36 kDa, 51-59 kDa
GenBank蛋白编号BC017296
基因名称 SESN3
Gene ID (NCBI) 143686
RRIDAB_2185602
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58005
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

实验方案

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.

Authors - Chia-Chen Chen
  • KD Validated
mouseIHC

Proc Natl Acad Sci U S A

FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.

Authors - Kwang Il Lee
mouseWB

Cell Death Dis

Pifithrin-μ sensitizes mTOR-activated liver cancer to sorafenib treatment

Authors - Jiarui Lv
humanWB

Elife

Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.

Authors - Veronique Nogueira
  • KD Validated
mouseWB

Biochim Biophys Acta Mol Basis Dis

Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner

Authors - Sujuan Liu
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
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