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SOLO Polyclonal antibody

SOLO Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23911-1-AP

Print datasheet

Synonyms

SESTD1, SEC14 domain and spectrin repeat-containing protein 1, SEC14 and spectrin domains 1, Protein Solo, Huntingtin-interacting protein-like protein

验证数据展示

  • WB analysis using 23911-1-AP

    WB analysis using 23911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23911-1-AP (SOLO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 23911-1-AP

    IP experiment of mouse brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 4ug; Detection:23911-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • IF Staining of HepG2 using 23911-1-AP

    IF Staining of HepG2 using 23911-1-AP

    Immunofluorescent analysis of HepG2 cells using 23911-1-AP (SOLO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of rat brain using 23911-1-AP

    IP experiment of rat brain using 23911-1-AP

    IP result of anti-SOLO (IP:23911-1-AP, 0ug; Detection:23911-1-AP 1:4000) with rat brain tissue lysate 1120 ug.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23911-1-AP targets SOLO in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SOLO fusion protein Ag21016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC14 and spectrin domains 1
Synonyms SESTD1, SEC14 domain and spectrin repeat-containing protein 1, SEC14 and spectrin domains 1, Protein Solo, Huntingtin-interacting protein-like protein
Calculated Molecular Weight 696 aa, 79 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC047578
Gene Symbol SOLO
Gene ID (NCBI) 91404
RRIDAB_2879360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86VW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SOLO antibody 23911-1-APDownload protocol
IF protocol for SOLO antibody 23911-1-APDownload protocol
IP protocol for SOLO antibody 23911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols