• Featured Product
  • KD/KO验证

SETD1B Polyclonal antibody

SETD1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 55005-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

Lysine N-methyltransferase 2G, KMT2G, KIAA1076, hSET1B, Histone-lysine N-methyltransferase SETD1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain tissue, MCF-7 cells, mouse liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55005-1-AP targets SETD1B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing 1B
别名 Lysine N-methyltransferase 2G, KMT2G, KIAA1076, hSET1B, Histone-lysine N-methyltransferase SETD1B
计算分子量 209 kDa
观测分子量 250-260 kDa, 450 kDa
GenBank蛋白编号NM_015048
基因名称 SETD1B
Gene ID (NCBI) 23067
RRIDAB_10858389
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

实验方案

Product Specific Protocols
WB protocol for SETD1B antibody 55005-1-APDownload protocol
IHC protocol for SETD1B antibody 55005-1-APDownload protocol
IF protocol for SETD1B antibody 55005-1-APDownload protocol
IP protocol for SETD1B antibody 55005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

EMBO J

Histone methyltransferases MLL2 and SETD1A/B play distinct roles in H3K4me3 deposition during the transition from totipotency to pluripotency

Authors - Jingjing Zhang
  • KD Validated
humanWB

Int J Mol Sci

(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.

Authors - Chen Chen
humanWB,IHC

Am J Cancer Res

Risk model-guided identification of MTDH expression as a marker for ferroptosis induction therapy in head and neck squamous cell carcinoma

Authors - Xueying Wang
mouseIHC

Biogerontology

Histone lysine methyltransferases and their specific methylation marks show significant changes in mouse testes from young to older ages

Authors - Yesim Bilmez
mouseIHC

Histochem Cell Biol

Expression of the histone lysine methyltransferases SETD1B, SETDB1, SETD2, and CFP1 exhibits significant changes in the oocytes and granulosa cells of aged mouse ovaries.

Authors - Yesim Bilmez
mouseWB,IHC,ChIP

Int Immunopharmacol

NF-κB p65 and SETDB1 expedite lipopolysaccharide-induced intestinal inflammation in mice by inducing IRF7/NLR-dependent macrophage M1 polarization

Authors - Li Chen
Loading...