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验证数据展示

  • WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

    WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

    WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

    FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug SETD8 Polyclonal antibody (14063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SETD8 Polyclonal antibody, PBS Only

SETD8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14063-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14063-1-PBS targets SETD8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SETD8 fusion protein Ag5114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SET domain containing (lysine methyltransferase) 8
别名
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC050346
基因名称 SETD8
Gene ID (NCBI) 387893
RRIDAB_2878006
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQR1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.