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  • Featured Product
  • KD/KO Validated

SETD8 Polyclonal antibody, PBS Only

SETD8 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14063-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

    WB analysis using 14063-1-AP (same clone as 14063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    IHC staining of human breast cancer using 14063-1-AP (same clone as 14063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14063-1-AP (SETD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

    WB analysis of RAW 264.7 using 14063-1-AP (same clone as 14063-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14063-1-AP (SETD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

    FC experiment of HEK-293T using 14063-1-AP (same clone as 14063-1-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug SETD8 Polyclonal antibody (14063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 14063-1-PBS in a different storage buffer formulation.

产品信息

14063-1-PBS targets SETD8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SETD8 fusion protein Ag5114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SET domain containing (lysine methyltransferase) 8
Synonyms
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC050346
Gene Symbol SETD8
Gene ID (NCBI) 387893
RRIDAB_2878006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQR1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SETD8, also known as Set8, PR-Set7, or KMT5A, is a member of the SET domain family of histone lysine methyltransferases and, to date, the only known enzyme that catalyzes monomethylation of H4K20 in eukaryotes, which is involved in DNA damage response, mitotic condensation, and DNA replication. SETD8 is overexpressed in various types of tumors, such as bladder cancer, non-small cell lung carcinoma, and small cell lung carcinoma, and is associated with a shorter survival time for gastric, esophageal, and prostate cancer patients.