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  • Featured Product
  • KD/KO Validated

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11231-1-AP

Print datasheet

Synonyms

Histone-lysine N-methyltransferase SETDB1, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain

验证数据展示

  • WB analysis of Jurkat using 11231-1-AP

    WB analysis of Jurkat using 11231-1-AP

    WB result of SETDB1 antibody (11231-1-AP, 1:1000) with si-Control and si-SETDB1 transfected Jurkat cells.

  • WB analysis using 11231-1-AP

    WB analysis using 11231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11231-1-AP

    IHC staining of human lung cancer using 11231-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11231-1-AP (SETDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IP experiment of MCF-7 using 11231-1-AP

    IP experiment of MCF-7 using 11231-1-AP

    IP result of anti-SETDB1 (IP:11231-1-AP, 3ug; Detection:11231-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

  • WB analysis using 11231-1-AP

    WB analysis using 11231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11231-1-AP

    WB analysis of HeLa using 11231-1-AP

    WB result of SETDB1 antibody (11231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETDB1 transfected HeLa cells.

  • IF Staining of A431 using 11231-1-AP

    IF Staining of A431 using 11231-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (11231-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of A431 using 11231-1-AP

    FC experiment of A431 using 11231-1-AP

    1x10^6 A431 cells were intracellularly stained with 0.4 ug SETDB1 Polyclonal antibody (11231-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, human testis tissue, MCF-7 cells, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11231-1-AP targets SETDB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SETDB1 fusion protein Ag1725 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SET domain, bifurcated 1
Synonyms Histone-lysine N-methyltransferase SETDB1, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
RRIDAB_2186069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

实验方案

Product Specific Protocols
WB protocol for SETDB1 antibody 11231-1-APDownload protocol
IHC protocol for SETDB1 antibody 11231-1-APDownload protocol
IF protocol for SETDB1 antibody 11231-1-APDownload protocol
IP protocol for SETDB1 antibody 11231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
  • KO Validated
View Article
mouseWB,CoIP

Nature

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Authors - Jiadong Liu
View Article
mouse,humanWB

Nature

Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.

Authors - Gabriel K Griffin
  • KO Validated
View Article
mouseWB,CoIP,ChIP

Nature

KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements

Authors - Shang-Min Zhang
View Article
humanWB

Nature

Genome surveillance by HUSH-mediated silencing of intronless mobile elements.

Authors - Marta Seczynska
  • KD Validated
View Article
humanWB

Science

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.

Authors - Iva A Tchasovnikarova
  • KD Validated
View Article