验证数据展示

WB analysis of Jurkat using 11231-1-AP
WB result of SETDB1 antibody (11231-1-AP, 1:1000) with si-Control and si-SETDB1 transfected Jurkat cells.
WB analysis using 11231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11231-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11231-1-AP (SETDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SETDB1 antibody included
ChIP experiment of HeLa using 11231-1-AP
Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of SETDB1 Antibody (11231-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.
IP experiment of MCF-7 using 11231-1-AP
IP result of anti-SETDB1 (IP:11231-1-AP, 3ug; Detection:11231-1-AP 1:1000) with MCF-7 cells lysate 2500ug.
WB analysis using 11231-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11231-1-AP
WB result of SETDB1 antibody (11231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETDB1 transfected HeLa cells.
IP experiment of MCF-7 using 11231-1-AP
IP result of anti-SETDB1 (IP:11231-1-AP, 4ug; Detection:11231-1-AP 1:3000) with MCF-7 cells lysate 1720 ug.
IF Staining of A431 using 11231-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (11231-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A431 using 11231-1-AP
1x10^6 A431 cells were intracellularly stained with 0.4 ug SETDB1 Polyclonal antibody (11231-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Cat No. 11231-1-AP

产品说明书

引用文献(86)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA and More (2)

别名

Histone-lysine N-methyltransferase SETDB1, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (86)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, human testis tissue, MCF-7 cells, PC-3 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	A431 cells
Positive ChIP detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Chromatin immunoprecipitation (ChIP)	CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 21 publications below
WB	See 64 publications below
IHC	See 9 publications below
IF	See 12 publications below
IP	See 9 publications below
CoIP	See 4 publications below
ChIP	See 22 publications below

产品信息

11231-1-AP targets SETDB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ChIP
经测试反应性	human
文献引用反应性	human, mouse
免疫原	SETDB1 fusion protein Ag1725 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	SET domain, bifurcated 1
别名	Histone-lysine N-methyltransferase SETDB1, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain
计算分子量	143 kDa
观测分子量	170 kDa
GenBank蛋白编号	BC009362
基因名称	SETDB1
Gene ID (NCBI)	9869
RRID	AB_2186069
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q15047
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

实验方案

Product Specific Protocols
WB protocol for SETDB1 antibody 11231-1-AP	Download protocol
IHC protocol for SETDB1 antibody 11231-1-AP	Download protocol
IF protocol for SETDB1 antibody 11231-1-AP	Download protocol
IP protocol for SETDB1 antibody 11231-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nature METTL3 regulates heterochromatin in mouse embryonic stem cells. Authors - Wenqi Xu KO Validated View Article
mouse	WB,CoIP	Nature The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity. Authors - Jiadong Liu View Article
mouse,human	WB	Nature Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity. Authors - Gabriel K Griffin KO Validated View Article
mouse	WB,CoIP,ChIP	Nature KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements Authors - Shang-Min Zhang View Article
human	WB	Nature Genome surveillance by HUSH-mediated silencing of intronless mobile elements. Authors - Marta Seczynska KD Validated View Article
human	WB	Science GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells. Authors - Iva A Tchasovnikarova KD Validated View Article

验证数据展示

WB analysis of Jurkat using 11231-1-AP

WB analysis using 11231-1-AP

IHC staining of human lung cancer using 11231-1-AP

ChIP experiment of HeLa using 11231-1-AP

IP experiment of MCF-7 using 11231-1-AP

WB analysis using 11231-1-AP

WB analysis of HeLa using 11231-1-AP

IP experiment of MCF-7 using 11231-1-AP

IF Staining of A431 using 11231-1-AP

FC experiment of A431 using 11231-1-AP

SETDB1 Polyclonal antibody

SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

METTL3 regulates heterochromatin in mouse embryonic stem cells.

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.

KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements

Genome surveillance by HUSH-mediated silencing of intronless mobile elements.

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.