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SETDB1 Monoclonal antibody, PBS Only

SETDB1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6E5

Cat No : 66293-1-PBS

Print datasheet

Synonyms

ESET, H3 K9 HMTase 4, H3 K9 HMTase4, KG1T, KIAA0067, KMT1E, Lysine N methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1, SETDB1

验证数据展示

  • WB analysis using 66293-1-Ig (same clone as 66293-1-PBS)

    WB analysis using 66293-1-Ig (same clone as 66293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66293-1-Ig (SETDB1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66293-1-Ig (same clone as 66293-1-PBS)

    IHC staining of mouse colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IHC staining of human colon using 66293-1-Ig (same clone as 66293-1-PBS)

    IHC staining of human colon using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66293-1-Ig (SETDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IF Staining of A431 using 66293-1-Ig (same clone as 66293-1-PBS)

    IF Staining of A431 using 66293-1-Ig (same clone as 66293-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (66293-1-Ig, Clone: 1H6E5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66293-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.

产品信息

66293-1-PBS targets SETDB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SETDB1 fusion protein Ag21644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SET domain, bifurcated 1
Synonyms ESET, H3 K9 HMTase 4, H3 K9 HMTase4, KG1T, KIAA0067, KMT1E, Lysine N methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1, SETDB1
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15047
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).