SETDB1 Recombinant antibody
SETDB1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240805D7
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, T-47D cells |
Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83772-5-RR targets SETDB1 in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | SETDB1 fusion protein Ag1725 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SET domain, bifurcated 1 |
Synonyms | Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366 |
Calculated Molecular Weight | 143 kDa |
Observed Molecular Weight | 170-180 kDa |
GenBank Accession Number | BC009362 |
Gene Symbol | SETDB1 |
Gene ID (NCBI) | 9869 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q15047 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).
实验方案
Product Specific Protocols | |
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WB protocol for SETDB1 antibody 83772-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |