• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human colon cancer using 83772-6-RR (same clone as 83772-6-PBS)

    IHC staining of human colon cancer using 83772-6-RR (same clone as 83772-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83772-6-RR (SETDB1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SETDB1 antibody included
  • IF Staining of HepG2 using 83772-6-RR (same clone as 83772-6-PBS)

    IF Staining of HepG2 using 83772-6-RR (same clone as 83772-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETDB1 antibody (83772-6-RR, Clone: 240805F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83772-6-RR (same clone as 83772-6-PBS)

    FC experiment of HeLa using 83772-6-RR (same clone as 83772-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SETDB1 Recombinant antibody (83772-6-RR, Clone:240805F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83772-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83772-6-RR

    Affinity and Kinetic Characterization of 83772-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83772-6-RR against Human SETDB1 were performed. The affinity constant is 0.18 nM.

SETDB1 Recombinant antibody, PBS Only

SETDB1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83772-6-PBS

Print datasheet

CloneNo. 240805F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy SETDB1 Ready-To-Use IHC Kit. SETDB1 primary antibody included.

产品信息

83772-6-PBS targets SETDB1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SETDB1 fusion protein Ag1725 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SET domain, bifurcated 1
Synonyms Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, ESET, ERG-associated protein with SET domain, EC:2.1.1.366
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC009362
Gene Symbol SETDB1
Gene ID (NCBI) 9869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15047
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).