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×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

    WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF1 antibody included
  • IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF1 antibody included

SF1 Polyclonal antibody, PBS Only

SF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11635-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SF1 Ready-To-Use IHC Kit. SF1 primary antibody included.

产品信息

11635-1-PBS targets SF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SF1 fusion protein Ag2209 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splicing factor 1
别名
计算分子量 638 aa, 68 kDa
观测分子量65-71 kDa
GenBank蛋白编号BC020217
基因名称 SF1
Gene ID (NCBI) 7536
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.