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SF1 Polyclonal antibody, PBS Only

SF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11635-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

    WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF1 antibody included
  • IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SF1 antibody included
Planning an IHC experiment? We recommend our IHCeasy SF1 Ready-To-Use IHC Kit. SF1 primary antibody included.

产品信息

11635-1-PBS targets SF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SF1 fusion protein Ag2209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 1
Synonyms
Calculated Molecular Weight 638 aa, 68 kDa
Observed Molecular Weight65-71 kDa
GenBank Accession NumberBC020217
Gene Symbol SF1
Gene ID (NCBI) 7536
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.