SF3B14 Polyclonal antibody
SF3B14 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12379-1-AP targets SF3B14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SF3B14 fusion protein Ag3045 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | splicing factor 3B, 14 kDa subunit |
| Synonyms | CGI 110, HSPC175, Ht006, P14, SAP14, SF3b 14 kDa subunit, SF3B14, SF3B14a |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC015463 |
| Gene Symbol | SF3B14 |
| Gene ID (NCBI) | 51639 |
| RRID | AB_2186517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3B4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pre-mRNA splicing by the human spliceosome requires the sequential recognition by RNA and protein factors of the 5′ and 3′ splice sites,the branch region and the polypyrimidine tract. This sequence is characterized by the stepwise association of the U1, U2, and U4/U5/U6 snRNP particles [PMID:9476892]. Human p14 (SF3b14), a component of the spliceosomal U2 snRNP, interacts directly with the pre-mRNA branch adenosine within the context of the bulged duplex formed between the pre-mRNA branch region and U2 snRNA. This association occurs early in spliceosome assembly and persists within the fully assembled spliceosome [PMID:21062891].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SF3B14 antibody 12379-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex. | ||
Genet Med de novo PHF5A variants are associated with craniofacial abnormalities, developmental delay, and hypospadias |