验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HEK-293 cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10482-1-AP targets SF3B4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SF3B4 fusion protein Ag0737 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor 3b, subunit 4, 49kDa |
| 别名 | SAP 49, SAP49, SF3B4, SF3b49, SF3b50, Splicing factor 3B subunit 4 |
| 计算分子量 | 44 aa, 3 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC004273 |
| 基因名称 | SF3B4 |
| Gene ID (NCBI) | 10262 |
| RRID | AB_2301639 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15427 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SF3B4 antibody 10482-1-AP | Download protocol |
| IHC protocol for SF3B4 antibody 10482-1-AP | Download protocol |
| IP protocol for SF3B4 antibody 10482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
J Mol Cell Cardiol Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy. | ||
Cell Death Dis SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.
| ||
bioRxiv Sf3b4 mutation in Xenopus tropicalis causes RNA splicing defects followed by massive gene dysregulation that disrupt cranial neural crest development |
