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验证数据展示

  • WB analysis of HEK-293 using 15614-1-AP

    WB analysis of HEK-293 using 15614-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15614-1-AP (SF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15614-1-AP

    IHC staining of human testis using 15614-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15614-1-AP (SF4 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15614-1-AP

    IF Staining of HepG2 using 15614-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 15614-1-AP (SF4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

SF4 Polyclonal antibody

SF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15614-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15614-1-AP targets SF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SF4 fusion protein Ag8006 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splicing factor 4
别名 SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858
计算分子量 72 kDa
观测分子量55 kDa, 72 kDa
GenBank蛋白编号BC002986
基因名称 SF4
Gene ID (NCBI) 57794
RRIDAB_10859818
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWZ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SF4 (Splicing factor 4), also known as SUGP1 (SURP and G-patch domain-containing protein 1) was first identified as a putative splicing factor based on domain composition analysis. SUGP1 protein is predicted to contain two SURP motifs, domains of alternative splicing regulators thought to be involved in RNA binding, as well as a G-patch domain, a region of six highly conserved glycine residues commonly found in eukaryotic RNA-processing proteins (PMID: 27206982). Research has shown that SUGP1 is involved in the regulation of cholesterol metabolism (PMID: 31474574).

实验方案

Product Specific Protocols
WB protocol for SF4 antibody 15614-1-APDownload protocol
IHC protocol for SF4 antibody 15614-1-APDownload protocol
IF protocol for SF4 antibody 15614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols