验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, mouse spleen tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
25256-1-AP targets SFMBT2 in WB, CoIP, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SFMBT2 fusion protein Ag19379 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Scm-like with four mbt domains 2 |
| 别名 | KIAA1617, SFMBT2 |
| 计算分子量 | 894 aa, 101 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC152430 |
| 基因名称 | SFMBT2 |
| Gene ID (NCBI) | 57713 |
| RRID | AB_2833000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VUG0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFMBT2 (Scm like with four mbt domains 2), also named as KIAA1617. It is located in Nucleus and ubiquitous expression in thyroid and ovary. The calculated molecular weight of SFMBT2 is 100 kDa. Although mammalian SFMBTs have similar structural features with PcG protein dSFMBT, biological function and target genes of SFMBT have been not investigated well. Lee K and Na W investigated biological function of human SFMBT2 that interacted with YY1 using AR (androgen receptor)-negative DU145 prostate cancer cells. They found that human SFMBT2 represses HOXB13 gene expression via its association with methylated histones H3 and H4 that are transcriptional repression marks. Furthermore, their findings indicate that SFMBT2 may be involved in regulation of cell growth of DU145 prostate cancer cells (PMID: 23385818).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SFMBT2 antibody 25256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer. | ||
Biol Direct Acetylation- and ubiquitination-regulated SFMBT2 acts as a tumor suppressor in clear cell renal cell carcinoma |
