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  • Featured Product
  • KD/KO Validated

SFRP1 Polyclonal antibody

SFRP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26460-1-AP

Print datasheet

Synonyms

FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1

验证数据展示

  • WB analysis using 26460-1-AP

    WB analysis using 26460-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26460-1-AP

    IF Staining of HeLa using 26460-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26460-1-AP (SFRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human breast cancer using 26460-1-AP

    IHC staining of human breast cancer using 26460-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SFRP1 antibody included
  • IHC staining of human breast cancer using 26460-1-AP

    IHC staining of human breast cancer using 26460-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SFRP1 antibody included
  • WB analysis using 26460-1-AP

    WB analysis using 26460-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 26460-1-AP

    IHC staining of human colon using 26460-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SFRP1 antibody included

经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, SH-SY5Y cells, U2OS cells, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SFRP1 Ready-To-Use IHC Kit. SFRP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26460-1-AP targets SFRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen SFRP1 fusion protein Ag24844 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted frizzled-related protein 1
Synonyms FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC036503
Gene Symbol SFRP1
Gene ID (NCBI) 6422
RRIDAB_2880522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N474
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFRP1 antibody 26460-1-APDownload protocol
IHC protocol for SFRP1 antibody 26460-1-APDownload protocol
IF protocol for SFRP1 antibody 26460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

APL Bioeng

Oscillatory shear stress-induced downregulation of TET1s injures vascular endothelial planar cell polarity by suppression of actin polymerization

Authors - Kai Qu
  • KD Validated
View Article
humanWB

Aging (Albany NY)

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Authors - Liansheng Sha
View Article
ratIHC

Cells

Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.

Authors - Keita Ipposhi
View Article
mouseWB

Stem Cell Rev Rep

Interleukin-19 in Bone Marrow Contributes to Bone Loss Via Suppressing Osteogenic Differentiation Potential of BMSCs in Old Mice

Authors - Enjun He
View Article
human,mouseWB

In Vitro Cell Dev Biol Anim

SFRP1 reduces neutrophil infiltration and inhibits the Wnt/β-catenin pathway to alleviate oral submucous fibrosis

Authors - Rong Zhou
View Article