SFRP2 Polyclonal antibody

SFRP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12189-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

sFRP-2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, SARP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 6 publications below

产品信息

12189-1-AP targets SFRP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SFRP2 fusion protein Ag2853 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secreted frizzled-related protein 2
别名 sFRP-2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, SARP1
计算分子量 295 aa, 34 kDa
观测分子量 33 kDa
GenBank蛋白编号BC008666
基因名称 SFRP2
Gene ID (NCBI) 6423
RRIDAB_2254520
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for SFRP2 antibody 12189-1-APDownload protocol
IF protocol for SFRP2 antibody 12189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.

Authors - Chenlin Zhou
humanWB

Front Oncol

Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression.

Authors - Danhui Ma
humanWB

Biomed Pharmacother

MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway.

Authors - Yuntao Guo
mouseWB

PLoS One

Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.

Authors - Zhang Xiujun X

Physiol Genomics

Tenotomy Immobilization as a Model to Investigate Skeletal Muscle Fibrosis (with Emphasis on Secreted Frizzled-Related Protein 2).

Authors - Uğur Akpulat
mouseWB

Psychiatry Investig

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis

Authors - Yue Ming
Loading...