• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SFRP4 Polyclonal antibody, PBS Only

SFRP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15328-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

    WB analysis of HEK-293 using 15328-1-AP (same clone as 15328-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    IP experiment of HeLa using 15328-1-AP (same clone as 15328-1-PBS)

    IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
  • IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of human breast cancer using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
  • IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

    IHC staining of rat testis using 15328-1-AP (same clone as 15328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15328-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFRP4 antibody included
Planning an IHC experiment? We recommend our IHCeasy SFRP4 Ready-To-Use IHC Kit. SFRP4 primary antibody included.

产品信息

15328-1-PBS targets SFRP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen SFRP4 fusion protein Ag6909 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted frizzled-related protein 4
Synonyms
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 41-51 kDa, 55 kDa
GenBank Accession NumberBC058911
Gene Symbol SFRP4
Gene ID (NCBI) 6424
RRIDAB_2187099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6FHJ7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.