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SFRP5 Polyclonal antibody

SFRP5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14283-1-AP

Print datasheet

Synonyms

SARP-3, SARP3, FRP-1b, FRP1B, Frizzled-related protein 1b

验证数据展示

  • WB analysis using 14283-1-AP

    WB analysis using 14283-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14283-1-AP (SFRP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 14283-1-AP

    IHC staining of human thyroid cancer using 14283-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 14283-1-AP

    IHC staining of mouse heart using 14283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14283-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 14283-1-AP

    IF Staining of A549 using 14283-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP5 antibody (14283-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHUVEC cells, L02 cells, mouse heart tissue, mouse retina tissue
Positive IHC detected inhuman thyroid cancer tissue, human cervical cancer tissue, mouse heart tissue, rat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14283-1-AP targets SFRP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SFRP5 fusion protein Ag5555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted frizzled-related protein 5
Synonyms SARP-3, SARP3, FRP-1b, FRP1B, Frizzled-related protein 1b
Calculated Molecular Weight 36 kDa
Observed Molecular Weight36-42 kDa
GenBank Accession NumberBC050435
Gene Symbol SFRP5
Gene ID (NCBI) 6425
RRIDAB_3085434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T4F7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Secreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes. SFRP5 is secreted by adipocytes and that it controls the micro-environment of white adipose tissue under conditions of metabolic stress (PMID: 20558665). SFRP5 gene consists of 317 amino acid residues and 3 coding exons and maps to chromosome 10q24.1 in humans. SFRP5 was initially found to be highly expressed in retinal pigment epithelium cells and moderately expressed in the pancreas. SFRP5 is involved in the pathological process of obesity, insulin resistance, dyslipidemia, inflammation, and fibrosis and is associated with obesity, T2D, and ASCVD (PMID: 31199714, PMID: 32004418).

实验方案

Product Specific Protocols
WB protocol for SFRP5 antibody 14283-1-APDownload protocol
IHC protocol for SFRP5 antibody 14283-1-APDownload protocol
IF protocol for SFRP5 antibody 14283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols