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验证数据展示

  • IHC staining of human cervical cancer using 19177-1-AP

    IHC staining of human cervical cancer using 19177-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19177-1-AP (SFRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 19177-1-AP

    IHC staining of human cervical cancer using 19177-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19177-1-AP (SFRP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SFRP5 Polyclonal antibody

SFRP5 Polyclonal Antibody for IHC, ELISA
Cat No. 19177-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

Frizzled related protein 1b, FRP 1b, FRP1B, SARP 3, SARP3, sFRP 5, SFRP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman cervical cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19177-1-AP targets SFRP5 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SFRP5 fusion protein Ag5715 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted frizzled-related protein 5
Synonyms Frizzled related protein 1b, FRP 1b, FRP1B, SARP 3, SARP3, sFRP 5, SFRP5
Calculated Molecular Weight 36 kDa
GenBank Accession NumberBC050435
Gene Symbol SFRP5
Gene ID (NCBI) 6425
RRIDAB_3085575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T4F7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Secreted frizzled-related protein 5 (SFRP5) is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes. SFRP5 is secreted by adipocytes and that it controls the micro-environment of white adipose tissue under conditions of metabolic stress (PMID: 20558665). SFRP5 gene consists of 317 amino acid residues and 3 coding exons and maps to chromosome 10q24.1 in humans. SFRP5 was initially found to be highly expressed in retinal pigment epithelium cells and moderately expressed in the pancreas. SFRP5 is involved in the pathological process of obesity, insulin resistance, dyslipidemia, inflammation, and fibrosis and is associated with obesity, T2D, and ASCVD (PMID: 31199714, PMID: 32004418).

实验方案

Product Specific Protocols
IHC protocol for SFRP5 antibody 19177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols