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SFRS14 Polyclonal antibody

SFRS14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12514-1-AP

Print datasheet

Synonyms

KIAA0365, SFRS14

验证数据展示

  • WB analysis using 12514-1-AP

    WB analysis using 12514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12514-1-AP (SFRS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12514-1-AP

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12514-1-AP

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, LNCaP cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12514-1-AP targets SFRS14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SFRS14 fusion protein Ag3195 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 14
Synonyms KIAA0365, SFRS14
Calculated Molecular Weight 988 aa, 120 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC020586
Gene Symbol SFRS14
Gene ID (NCBI) 10147
RRIDAB_2184923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IX01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFRS14 antibody 12514-1-APDownload protocol
IHC protocol for SFRS14 antibody 12514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols