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验证数据展示

  • WB analysis using 12514-1-AP

    WB analysis using 12514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12514-1-AP (SFRS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12514-1-AP

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12514-1-AP

    IHC staining of human prostate cancer using 12514-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12514-1-AP (SFRS14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SFRS14 Polyclonal antibody

SFRS14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12514-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0365, SFRS14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, LNCaP cells, rat testis tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12514-1-AP targets SFRS14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SFRS14 fusion protein Ag3195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splicing factor, arginine/serine-rich 14
别名 KIAA0365, SFRS14
计算分子量 988 aa, 120 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC020586
基因名称 SFRS14
Gene ID (NCBI) 10147
RRIDAB_2184923
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IX01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFRS14 antibody 12514-1-APDownload protocol
IHC protocol for SFRS14 antibody 12514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols