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SFRS17A Polyclonal antibody

SFRS17A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13441-1-AP

Print datasheet

Synonyms

CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A

验证数据展示

  • WB analysis of HeLa using 13441-1-AP

    WB analysis of HeLa using 13441-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 13441-1-AP

    IHC staining of mouse cerebellum using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 13441-1-AP

    IHC staining of mouse cerebellum using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HT-1080 using 13441-1-AP

    IF Staining of HT-1080 using 13441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using SFRS17A antibody (13441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, human heart tissue, human kidney tissue, HEK-293T cells
Positive IHC detected inmouse cerebellum tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-1080 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13441-1-AP targets SFRS17A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SFRS17A fusion protein Ag4252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 17A
Synonyms CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A
Calculated Molecular Weight 695 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC028151
Gene Symbol SFRS17A
Gene ID (NCBI) 8227
RRIDAB_2184937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SFRS17A antibody 13441-1-APDownload protocol
IHC protocol for SFRS17A antibody 13441-1-APDownload protocol
IF protocol for SFRS17A antibody 13441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols