• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21212-1-AP

Print datasheet

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

验证数据展示

  • WB analysis using 21212-1-AP

    WB analysis using 21212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21212-1-AP (SFRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy SFRS2 Ready-To-Use IHC Kit. SFRS2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21212-1-AP targets SFRS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen SFRS2 fusion protein Ag15612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 2
Synonyms Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC070086
Gene Symbol SFRS2
Gene ID (NCBI) 6427
RRIDAB_3085645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01130
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS2, also named as PR264 and SC-35, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

实验方案

Product Specific Protocols
WB protocol for SFRS2 antibody 21212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols