• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SFRS2 Polyclonal antibody

SFRS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21212-1-AP

Print datasheet

Synonyms

Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264

验证数据展示

  • WB analysis using 21212-1-AP

    WB analysis using 21212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21212-1-AP (SFRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy SFRS2 Ready-To-Use IHC Kit. SFRS2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21212-1-AP targets SFRS2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen SFRS2 fusion protein Ag15612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 2
Synonyms Serine/arginine-rich splicing factor 2, SC-35, SC35, SC 35, Protein PR264
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC070086
Gene Symbol SFRS2
Gene ID (NCBI) 6427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01130
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS2, also named as PR264 and SC-35, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

实验方案

Product Specific Protocols
WB protocol for SFRS2 antibody 21212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols