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验证数据展示

  • WB analysis of Jurkat using 24705-1-AP

    WB analysis of Jurkat using 24705-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24705-1-AP

    IF Staining of HeLa using 24705-1-AP

    Immunofluorescent analysis of HeLa cells using 24705-1-AP (SFRS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SFRS8 Polyclonal antibody

SFRS8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24705-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

SFRS8, SWAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24705-1-AP targets SFRS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 SFRS8 fusion protein Ag19762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
别名 SFRS8, SWAP
计算分子量 951 aa, 105 kDa
观测分子量 130-150 kDa
GenBank蛋白编号BC136678
基因名称 SFRS8
Gene ID (NCBI) 6433
RRIDAB_2879682
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12872
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.

实验方案

Product Specific Protocols
WB protocol for SFRS8 antibody 24705-1-APDownload protocol
IF protocol for SFRS8 antibody 24705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Clin Transl Med

Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication.

Authors - Yuanjiao Zhang
View Article