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验证数据展示

  • WB analysis of HeLa using 19758-1-AP

    WB analysis of HeLa using 19758-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 19758-1-AP

    IHC staining of human pancreas using 19758-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 19758-1-AP

    IHC staining of human pancreas using 19758-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 40x lens).

SFT2D3 Polyclonal antibody

SFT2D3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19758-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SFT2 domain containing 3, SFT2D3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19758-1-AP targets SFT2D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SFT2 domain containing 3
别名 SFT2 domain containing 3, SFT2D3
计算分子量 22 kDa
观测分子量 20-22 kDa
GenBank蛋白编号NM_032740
基因名称 SFT2D3
Gene ID (NCBI) 84826
RRIDAB_10640432
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ587I9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFT2D3 belongs to the SFT2 family. SFT2D3 may be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.

实验方案

Product Specific Protocols
WB protocol for SFT2D3 antibody 19758-1-APDownload protocol
IHC protocol for SFT2D3 antibody 19758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols