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SFT2D3 Polyclonal antibody

SFT2D3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19758-1-AP

Print datasheet

Synonyms

SFT2 domain containing 3, SFT2D3

验证数据展示

  • WB analysis of HeLa using 19758-1-AP

    WB analysis of HeLa using 19758-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19758-1-AP (SFT2D3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 19758-1-AP

    IHC staining of human pancreas using 19758-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 19758-1-AP

    IHC staining of human pancreas using 19758-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 19758-1-AP (SFT2D3 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19758-1-AP targets SFT2D3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SFT2 domain containing 3
Synonyms SFT2 domain containing 3, SFT2D3
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberNM_032740
Gene Symbol SFT2D3
Gene ID (NCBI) 84826
RRIDAB_10640432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ587I9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFT2D3 belongs to the SFT2 family. SFT2D3 may be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.

实验方案

Product Specific Protocols
WB protocol for SFT2D3 antibody 19758-1-APDownload protocol
IHC protocol for SFT2D3 antibody 19758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols