验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11839-1-AP targets Surfactant protein D in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Surfactant protein D fusion protein Ag2461 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | surfactant protein D |
| 别名 | COLEC7, Collectin 7, Lung surfactant protein D, PSP D, PSPD, SFTP4, SFTPD, SP D, surfactant protein D |
| 计算分子量 | 375 aa, 38 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC022318 |
| 基因名称 | Surfactant protein D |
| Gene ID (NCBI) | 6441 |
| RRID | AB_2254351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35247 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Surfactant protein D antibody 11839-1-AP | Download protocol |
| IHC protocol for Surfactant protein D antibody 11839-1-AP | Download protocol |
| IP protocol for Surfactant protein D antibody 11839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway. | ||
Hum Cell Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells. | ||
Transl Pediatr Untargeted lipidomics of bronchopulmonary dysplasia induced by hyperoxia exposure in rats | ||
J Thorac Dis Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice | ||
Environ Pollut LRRK2 is involved in heat exposure-induced acute lung injury and alveolar type II epithelial cell dysfunction |