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SFXN3 Polyclonal antibody

SFXN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15156-1-AP

Print datasheet

Synonyms

Sideroflexin-3, sideroflexin 3, SFX3

验证数据展示

  • WB analysis of HeLa using 15156-1-AP

    WB analysis of HeLa using 15156-1-AP

    WB result of SFXN3 antibody (15156-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN3 transfected HeLa cells.

  • WB analysis using 15156-1-AP

    WB analysis using 15156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15156-1-AP

    IHC staining of mouse brain using 15156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15156-1-AP

    WB analysis using 15156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15156-1-AP

    IHC staining of mouse brain using 15156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15156-1-AP

    WB analysis using 15156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 15156-1-AP

    IF Staining of BxPC-3 using 15156-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 15156-1-AP (SFXN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inC6 cells, Neuro-2a cells, rat brain tissue, NIH/3T3 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15156-1-AP targets SFXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SFXN3 fusion protein Ag7203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sideroflexin 3
Synonyms Sideroflexin-3, sideroflexin 3, SFX3
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000124
Gene Symbol SFXN3
Gene ID (NCBI) 81855
RRIDAB_2185825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.

实验方案

Product Specific Protocols
WB protocol for SFXN3 antibody 15156-1-APDownload protocol
IHC protocol for SFXN3 antibody 15156-1-APDownload protocol
IF protocol for SFXN3 antibody 15156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.

Authors - Mariluz Soula
View Article
humanIHC

PeerJ

Identification of iron metabolism-related genes as prognostic indicators for papillary thyroid carcinoma: a retrospective study

Authors - Tiefeng Jin
View Article
human,mouseWB

Nucleic Acids Res

Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription

Authors - Jingke Xie
View Article
humanWB

Am J Respir Crit Care Med

Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function.

Authors - Brian J Sandri
View Article