SGCG Polyclonal antibody

SGCG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18102-1-AP

Print datasheet

Synonyms

35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE



经过测试的应用

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SGCG Ready-To-Use IHC Kit. SGCG primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18102-1-AP targets SGCG in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SGCG fusion protein Ag12402 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
Synonyms 35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC074777
Gene Symbol SGCG
Gene ID (NCBI) 6445
RRIDAB_2286186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGCG antibody 18102-1-APDownload protocol
IHC protocol for SGCG antibody 18102-1-APDownload protocol
IP protocol for SGCG antibody 18102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Skelet Muscle

Novel γ-sarcoglycan interactors in murine muscle membranes.

Authors - Tara C Smith
mouseIHC

PLoS One

Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.

Authors - Alison M Blain
humanIF

PLoS One

Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.

Authors - Narinder Janghra
humanIF

Hum Gene Ther Methods

Optimisation of internally deleted dystrophin constructs.

Authors - Mojgan Reza
mouseIHC

Mol Vis

Identification of HMX1 target genes: a predictive promoter model approach.

Authors - Boulling Arnaud A