• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 21614-1-AP

    WB analysis using 21614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21614-1-AP

    WB analysis using 21614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21614-1-AP (SGCZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse heart using 21614-1-AP

    IF Staining of mouse heart using 21614-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SGCZ antibody (21614-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SGCZ Polyclonal antibody

SGCZ Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 21614-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

ZSG1, Zeta-SG, Zeta-sarcoglycan, Zeta sarcoglycan

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse heart tissue, mouse brain tissue, rat heart tissue
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21614-1-AP targets SGCZ in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SGCZ fusion protein Ag16305 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sarcoglycan zeta
别名 ZSG1, Zeta-SG, Zeta-sarcoglycan, Zeta sarcoglycan
计算分子量 265 aa, 30 kDa
观测分子量30-40 kDa
GenBank蛋白编号BC125037
基因名称 SGCZ
Gene ID (NCBI) 137868
RRIDAB_3085662
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96LD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sarcoglycan zeta, also known as SGCZ, ZSG1, is part of the sarcoglycan complex. The sarcoglycan complex is part of the dystrophin-associated glycoprotein complex (DGC), which bridges the inner cytoskeleton and the extracellular matrix. SGCZ plays a role in the maintenance of striated muscle membrane stability. SGCZ was also found as a component of the vascular smooth muscle sarcoglycan complex. SGCZ was reduced at the membrane in muscular dystrophy (PMID: 12189167).

实验方案

Product Specific Protocols
WB protocol for SGCZ antibody 21614-1-APDownload protocol
IF protocol for SGCZ antibody 21614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols