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SGEF Polyclonal antibody

SGEF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12493-1-AP

Print datasheet

Synonyms

CSGEF, HMFN1864, SGEF

验证数据展示

  • WB analysis of PC-3 using 12493-1-AP

    WB analysis of PC-3 using 12493-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12493-1-AP

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12493-1-AP

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

12493-1-AP targets SGEF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsFC
Tested Reactivity human
Cited Reactivitymouse
Immunogen SGEF fusion protein Ag3198 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Src homology 3 domain-containing guanine nucleotide exchange factor
Synonyms CSGEF, HMFN1864, SGEF
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC016628
Gene Symbol SGEF
Gene ID (NCBI) 26084
RRIDAB_10896997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DR7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGEF antibody 12493-1-APDownload protocol
IHC protocol for SGEF antibody 12493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

PLoS One

The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis.

Authors - Samson Thomas T
View Article