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验证数据展示

  • WB analysis of PC-3 using 12493-1-AP

    WB analysis of PC-3 using 12493-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12493-1-AP (SGEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12493-1-AP

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 12493-1-AP

    IHC staining of human prostate cancer using 12493-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12493-1-AP (SGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SGEF Polyclonal antibody

SGEF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12493-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

CSGEF, HMFN1864, SGEF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

12493-1-AP targets SGEF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用FC
经测试反应性 human
文献引用反应性mouse
免疫原 SGEF fusion protein Ag3198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Src homology 3 domain-containing guanine nucleotide exchange factor
别名 CSGEF, HMFN1864, SGEF
计算分子量 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC016628
基因名称 SGEF
Gene ID (NCBI) 26084
RRIDAB_10896997
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGEF antibody 12493-1-APDownload protocol
IHC protocol for SGEF antibody 12493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

PLoS One

The guanine-nucleotide exchange factor SGEF plays a crucial role in the formation of atherosclerosis.

Authors - Samson Thomas T
View Article