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  • Featured Product
  • KD/KO Validated

SGK1 Polyclonal antibody

SGK1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23394-1-AP

Print datasheet

Synonyms

SGK, Serum/glucocorticoid-regulated kinase 1, Serine/threonine-protein kinase Sgk1, EC:2.7.11.1

验证数据展示

  • IHC staining of human pancreas using 23394-1-AP

    IHC staining of human pancreas using 23394-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with SGK1 antibody included
  • IHC staining of human pancreas using 23394-1-AP

    IHC staining of human pancreas using 23394-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with SGK1 antibody included
  • IF Staining of HEK-293 using 23394-1-AP

    IF Staining of HEK-293 using 23394-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGK1 antibody (23394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 23394-1-AP

    IP experiment of HEK-293 using 23394-1-AP

    IP result of anti-SGK1 (IP:23394-1-AP, 4ug; Detection:23394-1-AP 1:600) with HEK-293 cells lysate 1480ug.

经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SGK1 Ready-To-Use IHC Kit. SGK1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below
IHCSee 1 publications below

产品信息

23394-1-AP targets SGK1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SGK1 fusion protein Ag17888 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serum/glucocorticoid regulated kinase 1
Synonyms SGK, Serum/glucocorticoid-regulated kinase 1, Serine/threonine-protein kinase Sgk1, EC:2.7.11.1
Calculated Molecular Weight 431 aa, 49 kDa
GenBank Accession NumberBC001263
Gene Symbol SGK1
Gene ID (NCBI) 6446
RRIDAB_2879271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00141
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for SGK1 antibody 23394-1-APDownload protocol
IF protocol for SGK1 antibody 23394-1-APDownload protocol
IP protocol for SGK1 antibody 23394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Environ Health Perspect

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Authors - Rongyi Ye
  • KD Validated
View Article
human

FASEB J

Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure.

Authors - Huaxin Zhou
View Article
mouse

J Innate Immun

Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.

Authors - Yunzhi Liu
View Article
mouse

J Immunol

Serum- and Glucocorticoid-Inducible Kinase 1 Promotes Alternative Macrophage Polarization and Restrains Inflammation through FoxO1 and STAT3 Signaling.

Authors - Junling Ren
View Article
mouseIHC

J Transl Med

Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3

Authors - Xiangdong Li
View Article
human

Appl Biochem Biotechnol

Long Non-coding RNA ZFAS1 Regulates Fibrosis and Scortosis in the Cell Model of Diabetic Nephropathy Through miR-525-5p/SGK1 Axis

Authors - Langen Zhuang
View Article