验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27444-1-AP targets SGK196 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SGK196 fusion protein Ag24922 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase-like protein SgK196 |
| 别名 | POMK, Sugen kinase 196, Protein O-mannose kinase |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC101548 |
| 基因名称 | SGK196 |
| Gene ID (NCBI) | 84197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H5K3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SGK196, also known as POMK (protein O-mannose kinase), is an enzyme that plays a significant role in multiple biological processes. As a type II transmembrane protein, SGK196 consists of a cytoplasmic tail, one transmembrane helix, and a luminal domain (PMID: 31913260). It is widely expressed in tissues such as skeletal muscle, brain, heart, and kidney. SGK196 has been identified as a valuable marker for melanoma and has been found to suppress the metastasis of basal-like breast cancer cells, suggesting its role in tumorigenesis and cell survival.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SGK196 antibody 27444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |