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SGLT1 Polyclonal antibody

SGLT1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30861-1-AP

Print datasheet

Synonyms

SLC5A1, High affinity sodium-glucose cotransporter

验证数据展示

  • WB analysis using 30861-1-AP

    WB analysis using 30861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30861-1-AP

    WB analysis using 30861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 30861-1-AP

    IP experiment of mouse kidney using 30861-1-AP

    IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug.

  • IHC staining of mouse small intestine using 30861-1-AP

    IHC staining of mouse small intestine using 30861-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 30861-1-AP

    IHC staining of mouse small intestine using 30861-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue, mouse kidney tissue, mouse small intestine tissue, rat colon tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

30861-1-AP targets SGLT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, pig
Immunogen SGLT1 fusion protein Ag29338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 5 (sodium/glucose cotransporter), member 1
Synonyms SLC5A1, High affinity sodium-glucose cotransporter
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberNM_000343
Gene Symbol SGLT1
Gene ID (NCBI) 6523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13866
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).

实验方案

Product Specific Protocols
WB protocol for SGLT1 antibody 30861-1-APDownload protocol
IP protocol for SGLT1 antibody 30861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Food Funct

Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism

Authors - Zehua Liu
View Article
pigWB

J Anim Sci

The Inflammatory Injury of Porcine Small Intestinal Epithelial Cells Induced by Deoxynivalenol is Related to the Decrease of Glucose Transport

Authors - Xiaopeng Tang
View Article