验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Transfected |
| Positive IHC detected in | human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
14184-1-AP targets SGSH in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | SGSH fusion protein Ag5435 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | N-sulfoglucosamine sulfohydrolase |
| Synonyms | HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC047318 |
| Gene Symbol | SGSH |
| Gene ID (NCBI) | 6448 |
| RRID | AB_2270366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51688 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SGSH(N-sulphoglucosamine sulphohydrolase) is also named as HSS, sulphamidase and belongs to the sulfatase family. Sulfamidase is synthesized as a 62 kDa precursor protein, which is modified with mannose 6-phosphate (M6P) residues, allowing their recognition by mannose-6-phosphate receptors in the Golgi complex and ensuring transport to the endosomal/lysosomal system(PMID:21671382). It catalyzes the third step of degradation of glucosaminoglycans and is required for the degradation of heparan sulphate. Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A), also known as Sanfilippo syndrome A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SGSH antibody 14184-1-AP | Download protocol |
| IHC protocol for SGSH antibody 14184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
