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SGSH Polyclonal antibody

SGSH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14184-1-AP

Print datasheet

Synonyms

HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase

验证数据展示

  • WB analysis of HEK-293 using 14184-1-AP

    WB analysis of HEK-293 using 14184-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14184-1-AP (SGSH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14184-1-AP

    IHC staining of human pancreas cancer using 14184-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas cancer using 14184-1-AP

    IHC staining of human pancreas cancer using 14184-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14184-1-AP (SGSH Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, Transfected
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14184-1-AP targets SGSH in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen SGSH fusion protein Ag5435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-sulfoglucosamine sulfohydrolase
Synonyms HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC047318
Gene Symbol SGSH
Gene ID (NCBI) 6448
RRIDAB_2270366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SGSH(N-sulphoglucosamine sulphohydrolase) is also named as HSS, sulphamidase and belongs to the sulfatase family. Sulfamidase is synthesized as a 62 kDa precursor protein, which is modified with mannose 6-phosphate (M6P) residues, allowing their recognition by mannose-6-phosphate receptors in the Golgi complex and ensuring transport to the endosomal/lysosomal system(PMID:21671382). It catalyzes the third step of degradation of glucosaminoglycans and is required for the degradation of heparan sulphate. Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A), also known as Sanfilippo syndrome A.

实验方案

Product Specific Protocols
WB protocol for SGSH antibody 14184-1-APDownload protocol
IHC protocol for SGSH antibody 14184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Exp Eye Res

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Authors - Bo Jiang
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