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SGSH Polyclonal antibody

SGSH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28150-1-AP

Print datasheet

Synonyms

HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase

验证数据展示

  • WB analysis using 28150-1-AP

    WB analysis using 28150-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 28150-1-AP (SGSH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 28150-1-AP

    IHC staining of human pancreas cancer using 28150-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28150-1-AP (SGSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 28150-1-AP

    IHC staining of human pancreas cancer using 28150-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28150-1-AP (SGSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28150-1-AP targets SGSH in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen SGSH fusion protein Ag27769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name N-sulfoglucosamine sulfohydrolase
Synonyms HSS, MPS3A, SFMD, SGSH, Sulfoglucosamine sulfamidase, Sulphamidase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC047318
Gene Symbol SGSH
Gene ID (NCBI) 6448
RRIDAB_2881073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGSH antibody 28150-1-APDownload protocol
IHC protocol for SGSH antibody 28150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols