验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, L02 cells, mouse brain tissue, mouse testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
20825-1-AP targets SGSM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | SGSM3 fusion protein Ag14800 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small G protein signaling modulator 3 |
| 别名 | RUN and TBC1 domain-containing protein 3, Rab-GTPase-activating protein-like protein, RabGAPLP, Merlin-associated protein, Merlin associated protein |
| 计算分子量 | 749 aa, 85 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC008078 |
| 基因名称 | SGSM3 |
| Gene ID (NCBI) | 27352 |
| RRID | AB_10693680 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96HU1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IHC protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IF protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IP protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. | ||
Mediators Inflamm Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling. | ||
Cell Rep Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. | ||
J Cell Biol TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
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