• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27147-1-AP

    WB analysis using 27147-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27147-1-AP (SGSM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27147-1-AP

    IHC staining of human breast cancer using 27147-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27147-1-AP (SGSM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27147-1-AP

    IHC staining of human breast cancer using 27147-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27147-1-AP (SGSM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SGSM3 Polyclonal antibody

SGSM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27147-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HT-1376 cells, L02 cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27147-1-AP targets SGSM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SGSM3 fusion protein Ag25954 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small G protein signaling modulator 3
别名 MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3
计算分子量 749 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC008078
基因名称 SGSM3
Gene ID (NCBI) 27352
RRIDAB_2880774
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HU1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGSM3 antibody 27147-1-APDownload protocol
IHC protocol for SGSM3 antibody 27147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols