SH2B1 Polyclonal antibody
SH2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human ovary tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12226-1-AP targets SH2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, CoIP, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | SH2B1 fusion protein Ag2863 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SH2B adaptor protein 1 |
Synonyms | SH2B, SH2 domain-containing protein 1B, SH2 B, PSM, Pro-rich, PH and SH2 domain-containing signaling mediator |
Calculated Molecular Weight | 426 aa, 45 kDa |
Observed Molecular Weight | 80 kDa |
GenBank Accession Number | BC010704 |
Gene Symbol | SH2B1 |
Gene ID (NCBI) | 25970 |
RRID | AB_2877838 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NRF2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SH2B1 antibody 12226-1-AP | Download protocol |
IHC protocol for SH2B1 antibody 12226-1-AP | Download protocol |
IF protocol for SH2B1 antibody 12226-1-AP | Download protocol |
IP protocol for SH2B1 antibody 12226-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |