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SH2B1 Polyclonal antibody

SH2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12226-1-AP

Print datasheet

Synonyms

SH2B, SH2 domain-containing protein 1B, SH2 B, PSM, Pro-rich, PH and SH2 domain-containing signaling mediator

验证数据展示

  • WB analysis of HEK-293 using 12226-1-AP

    WB analysis of HEK-293 using 12226-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12226-1-AP

    IP experiment of HEK-293 using 12226-1-AP

    IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:600) with HEK-293 cells lysate 3000ug.

  • IHC staining of human ovary cancer using 12226-1-AP

    IHC staining of human ovary cancer using 12226-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12226-1-AP (SH2B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12226-1-AP

    IF Staining of HeLa using 12226-1-AP

    Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12226-1-AP targets SH2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SH2B1 fusion protein Ag2863 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH2B adaptor protein 1
Synonyms SH2B, SH2 domain-containing protein 1B, SH2 B, PSM, Pro-rich, PH and SH2 domain-containing signaling mediator
Calculated Molecular Weight 426 aa, 45 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC010704
Gene Symbol SH2B1
Gene ID (NCBI) 25970
RRIDAB_2877838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).

实验方案

Product Specific Protocols
WB protocol for SH2B1 antibody 12226-1-APDownload protocol
IHC protocol for SH2B1 antibody 12226-1-APDownload protocol
IF protocol for SH2B1 antibody 12226-1-APDownload protocol
IP protocol for SH2B1 antibody 12226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF,CoIP

Redox Biol

Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation.

Authors - Xiaojuan Han
View Article