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SH2D2A Polyclonal antibody

SH2D2A Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 18539-1-AP

Print datasheet

Synonyms

T cell-specific adapter protein, SH2 domain-containing protein 2A, SH2 domain-containing adapter protein, SH2 domain protein 2A, SCAP

验证数据展示

  • WB analysis of K-562 using 18539-1-AP

    WB analysis of K-562 using 18539-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 18539-1-AP

    IHC staining of human heart using 18539-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 18539-1-AP

    IHC staining of human heart using 18539-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of K-562 using 18539-1-AP

    FC experiment of K-562 using 18539-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug SH2D2A Polyclonal antibody (18539-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18539-1-AP targets SH2D2A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SH2D2A fusion protein Ag13355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH2 domain protein 2A
Synonyms T cell-specific adapter protein, SH2 domain-containing protein 2A, SH2 domain-containing adapter protein, SH2 domain protein 2A, SCAP
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC012107
Gene Symbol SH2D2A
Gene ID (NCBI) 9047
RRIDAB_10640158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH2D2A antibody 18539-1-APDownload protocol
IHC protocol for SH2D2A antibody 18539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mult Scler Relat Disord

Downregulation of lncRNA XIST may promote Th17 differentiation through KDM6A-TSAd pathway in neuromyelitis optica spectrum disorders

Authors - Ruo-Yi Guo
View Article