• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 15641-1-AP

    WB analysis of HeLa using 15641-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 15641-1-AP

    IHC staining of human ovary cancer using 15641-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15641-1-AP

    IF Staining of HeLa using 15641-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

SH2D3A Polyclonal antibody

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15641-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15641-1-AP targets SH2D3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SH2D3A fusion protein Ag8132 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 3A
别名 UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1
计算分子量 576 aa, 63 kDa
观测分子量 64 kDa
GenBank蛋白编号BC006281
基因名称 SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

实验方案

Product Specific Protocols
WB protocol for SH2D3A antibody 15641-1-APDownload protocol
IHC protocol for SH2D3A antibody 15641-1-APDownload protocol
IF protocol for SH2D3A antibody 15641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols