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SH2D3A Polyclonal antibody

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15641-1-AP

Print datasheet

Synonyms

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

验证数据展示

  • WB analysis of HeLa using 15641-1-AP

    WB analysis of HeLa using 15641-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15641-1-AP

    IF Staining of HeLa using 15641-1-AP

    Immunofluorescent analysis of HeLa cells using 15641-1-AP (SH2D3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 15641-1-AP

    IHC staining of human ovary cancer using 15641-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15641-1-AP targets SH2D3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SH2D3A fusion protein Ag8132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH2 domain containing 3A
Synonyms UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1
Calculated Molecular Weight 576 aa, 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC006281
Gene Symbol SH2D3A
Gene ID (NCBI) 10045
RRIDAB_2286019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

实验方案

Product Specific Protocols
WB protocol for SH2D3A antibody 15641-1-APDownload protocol
IHC protocol for SH2D3A antibody 15641-1-APDownload protocol
IF protocol for SH2D3A antibody 15641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols