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验证数据展示

  • WB analysis using 17691-1-AP

    WB analysis using 17691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 17691-1-AP

    IP experiment of mouse lung using 17691-1-AP

    IP result of anti-SH3BP4 (IP:17691-1-AP, 4ug; Detection:17691-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • IF Staining of HeLa using 17691-1-AP

    IF Staining of HeLa using 17691-1-AP

    Immunofluorescent analysis of HeLa cells using 17691-1-AP (SH3BP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SH3BP4 Polyclonal antibody

SH3BP4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17691-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF/ICC, IP, ELISA

别名

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17691-1-AP targets SH3BP4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, pig
免疫原 SH3BP4 fusion protein Ag11847 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3-domain binding protein 4
别名 BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP
计算分子量 963 aa, 108 kDa
观测分子量 120 kDa
GenBank蛋白编号BC057396
基因名称 SH3BP4
Gene ID (NCBI) 23677
RRIDAB_2189364
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0V3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BP4 antibody 17691-1-APDownload protocol
IF protocol for SH3BP4 antibody 17691-1-APDownload protocol
IP protocol for SH3BP4 antibody 17691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Mol Med

SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF.

Authors - Kyu-Han Kim
  • KD Validated
View Article
pigWB

J Appl Physiol (1985)

Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs.

Authors - Agus Suryawan
View Article

Nat Commun

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival

Authors - Joanne Watson
View Article