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验证数据展示

  • WB analysis using 68045-1-Ig

    WB analysis using 68045-1-Ig

    Various cell lysates were subjected to SDS PAGE followed by western blot with 68045-1-Ig (SH3BP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

SH3BP4 Monoclonal antibody

SH3BP4 Monoclonal Antibody for WB, ELISA
Cat No. 68045-1-Ig

产品说明书

CloneNo. 2G4H3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, U2OS cells, A549 cells, HeLa cells, HepG2 cells, hTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68045-1-Ig targets SH3BP4 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 SH3BP4 fusion protein Ag31238 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SH3-domain binding protein 4
别名 BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP
计算分子量 963 aa, 108 kDa
观测分子量110 kDa
GenBank蛋白编号BC057396
基因名称 SH3BP4
Gene ID (NCBI) 23677
RRIDAB_2918787
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9P0V3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BP4 antibody 68045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols